Use of mass spectrometry-based profiling to evaluate the response of cells to biopolymer surfaces: protein expression on hyaluronic acid-modified surface

摘要

The cell responses to biopolymer surface at the early adhesion stages can be critical for cell survival. The purpose of this research was todevelopthe formation of hyaluronic acid (HA) biopolymer surface, and thefibroblasts were used as a experimental model to evaluatethe responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profiling system. Surfaces were covered by multi-walled carbon nanotubes(CNT), chitosan (CS), and HA to increase the surface area, enhance the adhesion of biopolymer and promote the rate of cell proliferation. The amount of adhered fibroblasts on CNT/CS/HA electrodes of quartz crystal microbalance (QCM) were greatly exceeded those on other surfaces that were consistent with cell-count technique. Moreover, analyzing differential protein expression of adhered fibroblasts on those biopolymer surfaces by proteomicapproaches identified CD36, CD44, PP2A, and CDK9 as key proteins. To further validate the efficacy of aforementioned proteins, antibody blockage treatments for those proteins were performed and distinguished by QCM and microscopic observations. As expected, the weights and densities of adhered fibroblasts were decreased. The application of utilizing mass spectrometry-based proteomicsin evaluation of cell adhesion on biopolymer was proposed.