Absolute Quantification of Prion Protein (90-231) Using Stable Isotopelabeled chymotryptic Peptide Standards in a nanoLC-MRM Workflow

摘要

1. Digestion of PrP with chymotrypsin produces peptides suitable for a nanoLC-MRM AQUA assay. (1) Lacking chemically active residues (2) LOQ = 80 amol LOD = 20 - 40 amol Dynamic range over 3.5 orders of magnitude (3) YRPVDQY sequence is identical in 80 % of species surveyed, whereas VVEQMCTTQYQK is identical in only 5 % of species surveyed.