SNO-SNIPE(R): A Surface-functionalized Nanoprobe-based Identification Strategy for Protein S-Nitrosylation Enrichment

摘要

S-nitrosylation, specifically targeting on thiol group of cysteine residues on proteins by nitric oxide, is an important post-translational modification involving in cardiovascular regulation and immune response. Due to its labile nature and low abundance, detection of S-nitrosylated proteins remains methodologically challenging. To date, most methods are indirect enrichment via chemical replacement on S-nitrosylation sites, such as biotin switch method, which, however, suffers from low sample recovery, false-positive biotinylation signal and complex reaction scheme. Here, we report a magnetic nanoprobe-based affinity method for direct and site-specific enrichment and identification of the S-nitrosylated proteins. The magnetic nanoprobe allows simple one-step isolation by magnet. With enhanced sensitivity, we expect that such dataset will facilitate in-depth analysis of the potential motifs.