Increased throughput for label-free quantitative proteomics by combining data-independent acquisition with accelerated online 2D-LC

摘要

Compared to traditional methods our accelerated approach achieved excellent qualitative and absolute quantitative reproducibility in less than half the time. 1.5 days of valuable instrument time was saved comparing 2 conditions in triplicate by online 5 fraction 2D-RP/RP LC-MS. DIA/MS-E combined with accelerated online 2D-LC provides both depth and significantly increased throughput for discovery, quantification and validation experiments.