The Study of Ion Transmission of Macromolecules Using Multiple Quadrupoles Instrument Coupled with MALDI Ion Source

摘要

Most ion soft landing (SL) studies use electrospray ionization (ESI) or electrosonic spray ionization (ESSI) source as their ion sources. However, when macromolecules such as proteins or protein complexes are analyzed, the high charge numbers make the mass spectra very complicated and therefore cause the ion selection difficult. Moreover, ion transmission of most ion soft landing instruments is limited to m/z value less than 4,000. In this study, we used matrix-assisted laser desorption/ionization (MALDI) as an ion source and used homemade multiple quadrupoles system to guide high mass MALDI ions, e.g. cytochrome c (CyctC), into the quadrupoles system and the mass analyzer.