Label-free quantitative proteomic analysis of Experimental autoimmune myocarditis induced in the rat

摘要

Myocarditis is a cardiac disease associated with inflammation and injury of the myocardium. Label-free quantitative proteomic technology now allows us to examine global alterations in protein expression in the myocarditis and provide new biomarkers into diagnosis involved in cardiac dysfunction. The use of proteomics to investigate myocarditis should result in the generation of new diagnostic and therapeutic markers. In this study, we separated by 1-DE, followed by tryptic digestion and the analysis of differential expressed peptides via nano-UPLC and nano electrospray ionization-Q-TOF mass spectrometry (nano-ESI/Q/TOF MS). The PLGS search program, which searched against the Uniprot database, was used for peptide sequence identification and quantitation. We were normalized by predigested yeast enolase. We analyzed proteins of heart tissues in control and 16 days (medium inflammation) rats, and identified 120 proteins with increased expression including glucose regulated protein (GRP)78, heat shock protein (HSP)90beta and calreticulin, and 181 proteins with decreased expression. It is suggested that in the EAM model, Unfolded protein response play a role in the protection or deterioration of the heart and may be new targets for treatment.