Proteomic Discovery of Gastric Cancer Tissue Biomarkers Using MALDI-IMS and 2D-DIGE

摘要

Without early dingoes and treatment, gastric cancer (GC) has a significant higher rate of morbidity and mortality. Therefore, the early precise detection of gastric cancer is considered to be the best treatment for prolonging survival of patients with GC. Thus, to search and identify reliable biomarkers of gastric cancers coupled with more efficient early diagnostic methods is critical and urgent for preventing tumor metastasis and improving therapeutic success. Our studies reveal several putative biomarkers of GC including HNP-1, -2, -3 and GRP78 by using MALDI-TOF/TOF-MS. After carefully characterizations and assessments, we demonstrated that HNP-1, -2 and 3 derived from tumor associated neutrophils depositing onto gastric tumors may serve as good putative biomarkers of gastric cancers and GRP78 is decorating molecules on the GC cell membrane. Furthermore, in order to evaluate the capability of GRP78 acting as a drug-targeting protein in GC, we developed an antitumor drug delivery system carrying GRP78-binding peptide and then characterized its delivery efficiency in xenografts animal model. Our in vivo and in vitro data suggest that GRP78 is a good therapeutic targeting molecule for gastric cancer. Summarizing our studies, we currently define putative GC biomarkers including HNP-1-2 and -3 and a novel protein, GRP78 for drug targeting to GC cells. Both discoveries are extremely important for diagnosis and treatments of patients with GC in the form of early stage.