Lipid Profile of a Mouse Model of Blast Induced Traumatic Brain Injury.

摘要

Combat related blasts generate atmospheric pressure changes resulting in non penetrating blast induced traumatic brain injury (bTBI) with physical and psychological consequences. Lipids, a major component of brain tissue account for half of the brain dry weight. They play an important role in cell signaling and cell death. Altered levels of lipids in brain tissue are associated with neuronal diseases such as Alzheimer's, and Niemann-Pick disease. In the present work we used MALDI-MS for direct analysis and imaging of lipids in tissue a mouse model to study the consequences of exposure to blast. Caprioli developed the field of in situ analysis of biomolecules by imaging peptides and proteins in normal and diseased tissue. In this work we used new matrices we developed to advance the field of lipid imaging and were able to generate detailed structural information with histological accuracy. After mapping the content and distribution of lipids in the normal brain, we have demonstrated the changes that occur in the lipid make up of the brain in blast induced traumatic brain injury (bTBI).