Identification of Protein Isoforms in Fungi by Mass Spectrometry Analyses Using Alternative Splicing Databases

摘要

Dozens of new peptides were detected by using the alternative splicing databases in both A. flavus and M. oryzae. The alternative splicing database approach also showed potential to improve genome annotation. Applications of multiple search engines effectively reduced the possible false positive results and validated the protein identifications from the alternative splicing database. Applying the appropriate constraints reduced the variation between alignment programs and predicted the most significant isoforms.