Developing a high throughput and standardized LC-MS/MS method for steroid quantification in human serum

摘要

The method has been developed and validated for a LC-MS system consisting of a CTC PAL autosampler, an Agilent HPLC 1100/1200 binary pump, an Agilent column oven and ABSciex 4000 / 4000QTRAP mass spectrometer with ESI source. Optionally the ABSciex 5500QTRAP mass spectrometer can be used to improved the sensitivity. Systems from other manufacturers (Shimadzu, Waters, Thermo) have been also tested and will be validated. The described workflow and estimated time scale is applied for a full 96-well plate configuration (i.e. 80 samples). With smaller number of samples (1/2 plate for 36 samples or 1/3 plate for 20 samples) the measurement time will be reduced accordingly. The 16 steroid hormones pannel is presented in Stero/DQ~(~R) Kit~(*) and is the most versatile LC-MSMS based platform to deliver high-throughput and accurate quantitative steroid measurement, highly suitable for clinical metabolomics.