Expansion of the MDM2 Interactome Using Quantitative Mass Spectrometry Data and A Screen for Linear/Disordered Motifs

摘要

Quantitative MS provides a snapshot of the changes occuring in cells after stress or drug treatment, and is used in this study to identify proteins and pathways influenced by the MDM2 modulating drug Nutlin. Consensus peptide motifs can be used to filter on proteomic datasets, and here new MDM2 binding peptides and a full length MDM2 binding protein were identified using this method. It was discovered that MDM2 may have distinct mechanisms of peptide binding at the N-terminal domain. Future work will be to further investigate this mechanism, and continue to validate other proteomic MS results.