Functional Polymer Membranes for Protein Digestion and Peptide Separation Prior to Analysis by Mass Spectrometry

摘要

Trypsin immobilized in membrane pores allows faster protein digestion with higher sequence coverage and better SDS compatibility than in-solution digestion. Anionic polymer brushes fractionate peptides according to their pI. Tandem membrane discs with different functional groups enrich both mono- and multiply phosphorylated peptides from complex protein digests.