Identification of 1-deoxy-sphingoid bases and N-acyl-1-deoxy-sphingoid bases by LC-ESI-MS/MS

摘要

A novel sphingoid base, 1-deoxysphinganine, was recently reported1 to accumulate in fumonisin B_(1) (FB_(1)) treated cells in culture and in livers and kidneys from mice exposed to this mycotoxin. Here we developed high-throughput, structure-specific, and quantitative analysis of sphingolipids by high performance liquid chromatography tandem mass spectrometry (HPLC MS/MS) and a fast, sensitive, high accuracy, high resolution method based on a NanoAcuity ultra performance liquid chromatography (UPLC) coupled to an electrospray ionization (ESI) QSTAR Pulsar i time-of-flight (TOF) mass spectrometer.