EDD is a powerful tandem MS technique providing informative fragmentation while minimizing the loss of labile post translational modifications when applied to glycosaminoglycan carbohydrates. EDD allows for epimeric differentiation in GAG residues due to a proposed radical based fragmentation mechanism in heparan sulfates, but the differing glycosidic linkage found in chondroitin sulfates results in differed fragmentation pathways.
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