FRACTIONATING AND QUANTIFYING METHOD OF CHONDROITIN SULFATE PROTEOGLYCAN AND CHONDROITIN SULFATE GLYCOSAMINOGLYCAN

摘要

PROBLEM TO BE SOLVED: To provide a method that does not require a complex fractionation operation as in prior art, and in which chondroitin sulfate proteoglycan and chondroitin sulfate glycosaminoglycan can be simply and efficiently fractionated and quantified.;SOLUTION: A method for fractionating and quantifying chondroitin sulfate proteoglycan and chondroitin sulfate glycosaminoglycan includes: (1) a process of fractionating chondroitin sulfate proteoglycan and chondroitin sulfate glycosaminoglycan which can be present in a test sample by using a difference between dissolubility of chondroitin sulfate proteoglycan and dissolubility of chondroitin sulfate glycosaminoglycan to solvent; and (2) a process of decomposing chondroitin sulfate proteoglycan and chondroitin sulfate glycosaminoglycan which are obtained by fractionation into unsaturated disaccharide by chondroitinase, separating and quantifying each unsaturated disaccharide derived from chondroitin sulfate proteoglycan and derived from chondroitin sulfate glycosaminoglycan.;SELECTED DRAWING: Figure 1;COPYRIGHT: (C)2017,JPO&INPIT