Simultaneous Determination of Naloxone 6beta-naloxol, and Naloxone-3beta-D-glucuronide in Mouse Plasma Using Liquid Chromatography-tandem Mass Spectrometry

摘要

A single high-throughput, sensitive LC-MS/MS method for simultaneous quantitation of NLX, NLL, and NLG was developed and validated over the curve range from 0.200 to 100 ng/mL for NLX, from 0.400 to 200 ng/mL for NLL and from 0.500 to 259 ng/mL for NLG. The LLOQ for NLX, NLL and NLX were 0.200, 0.400 and 0.500 ng/mL, respectively. These values were similar to the LLOQ values determined using two different methods for NLX, NLL and NLG.