Profiling of N-acetylated protein termini provides in-depth insights into the N-terminal nature of the proteome

摘要

SCX can be used to analyze the N-acetylome and phosphoproteome simul-tanously. We identified 1391 N-acetylated and more that 5000 phosphorylated peptides from a HEK293 lysate using a multi enzyme approach Comparisons of different species reveals N-terminal biases that could be traced back to the genome level [5].