Identification of new phase I and phase II metabolites of antineoplastic agents in rat urine using HPLC/MS/MS

摘要

In total, 32 metabolites for dimefluron and 29 metabolites for benfluron were detected in the rat in vivo biotransformation based on the comparison of chromatograms of rat urine with placebo experiment, the support of UV chromatograms, extracted ion chromatograms for expected masses of metabolites together with the information about characteristic neutral losses. The oxidation, demethylation, carbonyl reduction, glucuronidation and sulfatation reactions are among observed mechanisms of the metabolite formation.