Investigation of Extraction Recovery for a Novel Anti-cancer Agent in Rat and Beagle Whole Blood

摘要

The final extraction methods for rat and dog blood are different, due to the natural endurance of the red blood cell between species. A protein precipitation or liquid-liquid extraction combined with additional cell disruption procedure was ultimately used for the rat and beagle whole blood assays. It has been demonstrated that the apparent instability was in fact a poor extraction recovery of the compound from the red blood cells. These two assays, simple and not time consuming, were developed and successfully validated for bio-analytical GLP studies.