Carboxyl Group Protein Footprinting for Mapping the Dimerization Interface and Phosphorylation-induced Conformational Changes of a Membrane-associated Protein Kinase

摘要

GEE protein mapping probes: (1) dimer interface, which showed increased "protection", (2) PTM-induced protein conformational changes. Her4 (ErbB-4), a receptor tyrosine kinase that is s mutated in malignant melanoma and lung cancer, dimerizes, autophosphorylates, and activates several intracellular signaling cascades following growth-factor binding.