Label free Differential Lipid Analysis for Rat Brain Samples Affected fy Stroke using a Linear Ion Trap Orbitrap Mass Spectrometer

摘要

1. ESI-MS/MS results showed similar results to MALDI-MS for the PC species and the more abundant lipid species. 2. ESI expts. conducted with stroke brain in comparison with control from a healthy rat brain showed more increased intensities of lipid species in control brain. 3. ESI expts. conducted with stroke brain in comparison with non-stroke portion of the stroke affected brain showed a surge in the lipid species in the stroke. 4. For MALDI analysis, the stroke affected areas showed disruption in lipid species compared to control portion of the brain. 5. DHA proved to be a better matrix for evaluating lipid species on the MALDI LTQ compared to the matrices currently available.