Differential Proteomic Analysis in 3 Different Types of Human Lymphomas Following Nutlin-3A-induced p53 Stabilization Activation

摘要

ICPL approach coupled with 1D-SDS-PAGE & nLC-MS/MS was applied for a large-scale proteomics analysis of 3 types of human lymphoma, providing successful identification & relative quantitation of differentially expressed proteins. Our findings provide important advances in understanding the biology of nutlin-3A treatment in wt p53 lymphoma cells, demonstrating significant known & novel protein changes. This study stresses out the biological similarities as well as differences between HL, MCL & ALCL. Further data processing will uncover far more comprehensive & thorough information on the participating pathways in haematological malignancies. Validation studies using genetic approaches are being performed, confirming a selection of abundantly expressed proteins of interest.