2D DIGE proteomics of rat mammary gland proteins to identify basis of anti-cancer activity by polyphenols

摘要

Of approximately 2000 protein spots detected in the 32 gel set, 162 spots were significantly different between day 21 and day 50 mammary gland (age effect; regardless of treatment); of these, 4 were identified (Table 1; Figure 2A shows an example of the age effect). Sixteen proteins were significantly different due to polyphenol treatment (treatment effect; regardless of age); of these, three were identified (Table 1; Figure 2B shows an example of the treatment effect). Nine spots were determined to have a significant interaction of age and treatment; of these, four were identified (Table 1; Figure 5 shows an example of the significant interaction). One hundred spots were missing at one age but not the other; of these, three were identified (Table 1; Figure 6 shows an example of a spot that was missing in 21-day samples, butpresent in 50-day samples). Thus far, the majority of the proteomic differences detected in whole rat mammary gland were age-effects.