Phospholipid Removal:- A Comparison between Traditional Liquid-liquid Extraction (LLE) and Supported Liquid Extraction (SLE) using LC-MS/MS Analysis

摘要

Low levels of lyso-PLs were present in both SLE+ and LLE extracts for most extraction solvents. The amount of lyso-PL extracted with 90/10 DCM/IPA increased with pH using SLE+ (to a maximum of 40percent of the PPT+ level). This was not observed with LLE. Low levels of PL's were seen using extration solvents of 98/2 hexane/3-methyl-1-butanol, MTBE and DCM for both LLE and SLE+. As the extraction solvent polarity increased, SLE+ showed higher levels of PL's compared to LLE. Highest levels were seen at acidic pHs. SLE+ does show increased levels of phospholipids when using more polar extraction solvents. However using traditional extraction conditions SLE+ and LLE provide comparable removal of phospholipids.