Application of a Novel GC-MS Method for Assessing Endogenous Metabolites in Exhaled Breath Condensate

摘要

The DE concentration in this study was set to simulate the exposure by a pedestrian at a typical urban intersection. The short term, low level exposure by ten healthy adults studied here did not result in significant changes in the patterns of metabolite concentration in EBC due to the exposure. However, exercising while participating in these experiments did lower the concentrations of 3-methylbutanal and 1-butanol. Significant random subject effects in metabolite concentration were seen for multiple compounds, suggesting potential difference in susceptibility for individuals. For some compounds, females had significantly lower levels of metabolites than males. In addition to the patterns presented here, the data collected from this study also provides background for future EBC work by building a library of normal EBC patterns that can be used with traditional source-specific biomarker measurements. Because this method is performed using thermal-desorption GC-MS, equipment that is relatively readily available, there is capability for this method to be replicated and applied to future studies.