Immobilized Peptide as Affinity Chromatography Ligand for Soybean Peroxidase Purification

摘要

Affinity chromatography (AC) is a valuable tool for the purification of proteins because it integrates several downstream steps, like isolation and specific purification, in only one step. Integrative procedures provide increasing yield and productivity at the industrial scale [1]. Discovery of biospecific ligands have a key role in AC development, phage display and chemical combinatorial libraries may lead to the development of tailor-made peptide ligands [2]. Successful affinity purification requires a biospecific ligand which can be covalently attached on a chromatographic support [3]. An affinity peptide ligand for the soybean peroxidase (SBP) was identified from the screening of a combinatorial library composed by 130,321 tetrapeptides.