Countercurrent chromatography (CCC), a liquid-liquid partitioning method carried out in flowthrough tubing coils mounted in planetary centrifuges, has served well in laboratory-scale preparative purification of small molecules, including peptides. A recent breakthrough in the design of the CCC rotor has enabled the use of aqueous-organic solvent systems containing the heavy alcohols (polar solvent systems) and the aqueous 2-phase solvent systems which extend the technique to the large molecules such as proteins, other polymers and many more types of peptides. Here we present application of the new spiral tubing support (STS) separation rotor to the preparative purification of peptides and proteins.
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