In the chemical synthesis of peptides, either a completely linear approach or a convergent approach may be followed. The latter approach, in which the final peptide is assembled from protected peptide fragments, is generally preferred for longer peptides, since overall yields are intrinsically higher and fragments may be prepared in parallel resulting in a shorter overall synthesis time. In a convergent synthesis, all fragments but the C-terminal fragment must be protected at their C-terminal with a protecting function that can be selectively cleaved. In case of peptide synthesis on a solid support, a handle on the support itself usually provides this function. However, for peptide synthesis on a manufacturing scale, solution-phase synthesis is preferred over solid-phase synthesis. Particularly preferred is a synthesis according to DioRaSSP~R, a solution-phase method which combines the advantages of the solid-phase and the classical solution-phase process [1].
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