A REGENERATIVE BIOSENSING SURFACE USING ELECTROCHEMICAL DESORPTION OF SELF-ASSEMBLED MONOLAYER IN MICROFLUIDICS

摘要

We report a novel method to regenerate a biosensor surface in microfluidics. By applying a low DC voltage (0.9V) between two electrodes, the sensing surface resets to be reusable/reconfigurable through electrodesorption of short-chain self assembled monolayers (SAMs). This method is free from electrolysis (HER (Hydrogen Evolution Reaction)) and electrode oxidation (peel-off). Real-time SPR (Surface Plasmon Resonance) measurements demonstrate streptavidin-bound COOH-SAM completely desorbs and CH_3-terminated SAM forms on the sensing surface to capture a target protein, fibrinogen, in a microfluidic device. The repeatability of the electrodesorption method is also presented.