Pregnancy rate of frozen-thawed pronucleate stages embryos from IVF and ICSI

摘要

The purpose of this study was to determine and compare the survival and pregnancy rate of frozen-thawed pronucleate stage embryos (PN) of IVF (n=191) and ICSI (n=240) patients. The mean number of obtained oocytes, fertilized, fertilization rate of IVF patients was (12.9+-5.7; [n=2458]; 8.9+-4.2, [n=1701], 69.2%). 1210 pronucleate stage embryos were frozen and 850 were thawed. 558 were survived (65.6% survival rate). This survived pronucleate stage embryos were cultured for 24 hours and transferred. 34 women became pregnant (17.8%). In ICSI program the mean number of obtained oocytes, fertilized, fertilization rate was (12.7+-5.3, [n= 3038]; 7.3+-2.8; [n=1755]; 57.8%). 1210 pronucleate oocytes embryos were frozen and 864 were thawed. However, only 592 were survived (68.5%). 22 pregnancies were achieved (9.2%; p=0.068). In both groups, the pronucleate stage embryos survival rate was 67.1% and the pregnancy rate was 13.6%. Conclusion: The survival and pregnancy rate was similar in both groups and added more than 10% pregnancy rate for ART program.