SYSTEMIC CYTOKINE PROFILES OF MICE VACCINATED WITH NAKED DNAS ENCODING SIX OPEN READING FRAME ANTIGENS OF PORCINE CIRCOVIRUS TYPE 2 (PCV2)

摘要

Porcine circovirus type 2 (PCV2) is associated with various disease syndromes in pigs, including postweaning multisystemic wasting syndrome (PMWS), proliferating and necrotizing pneumonia (PNP), porcine dermatitis and nephropathy syndrome (PDNS), porcine respiratory disease complex (PRDC), and abortion and reproductive problems. These syndromes are now grouped under the condition porcine circovirus disease (PCVD) (1). Vaccination with plasmid DNA encoding foreign antigens is one of the most significant advances that has been made in immunology and vaccinology in recent years. It has been shown that administration of the DNA by intramuscular immunization generally elicits cellular (Thl) immune responses, whereas particle bombardment by using a gene gun predominantly elicits antibody (Th2) responses. Here, we investigated the cellular immune responses generated by vaccinating mice intramuscularly (i.m.) with high quantities of each of the different PCV2 ORFs and then examining the cytokine profiles in their PBMCs seven and 35 days later.