Biofilm catalysts for decontamination of wastewater polluted by aniline were developed. Rhodococcus erythropolis, Ochrobactrum sp. and Comamonas sp. were identified as biodegraders utilizing aniline as the only C-source up to concentration 2.0 g/L, 2.5 g/L and 3.5 g/L, respectively. Influence of environmental conditions on the growth and biodegradation capacity of above mentioned strains was studied. The ability to adhere on the carrier surface was the key parameter in development of technological strain. Commercial material AnoxKaldnes-K1 was chosen as the carrier. The best coloni- zation of the carrier in continuous systems proved R. erythropolis. Biodegradation efficiency of prepared biocatalysts was investigated under standardized conditions in laboratory biofilm reactor. Results from laboratory experiments were verified in continu- ous pilot plant where industrial wastewater was applied. Efficiency of the fluidized bed reactor (100 L, carrier with R. erythropolis biofilm) expressed as biodegradation of dis- solved organic compounds was 90 % at residence time of 6 days. Received data were used for design of larger facilities (full-scale plant). The suggested process of wastewater treatment consists of 2 biofilm fluidized bed reactors enabling variable control.
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