A Portable Impedance Immunosensing System for Rapid Detection of Salmonella Typhimurium

摘要

Salmonella Typhimurium is one of the most dangerous foodborne pathogens and poses a significant threat to human health. The objective of this study was to develop a portable impedance immunosensing system for rapid and sensitive detection of S. Typhimurium in poultry. The developed portable impedance immunosensing system consisted of a gold interdigitated array microelectrode (IDAM), a signal acquisitive interface and a laptop computer with LabVIEWsoftware. The IDAM was first functionalized with 16-Mercaptohexadecanoic acid, and streptavidin was immobilized onto the electrode surface through covalent bonding. Then, biotin-labelled S. Typhimurium-antibody was immobilized onto the IDAM surface. Samples were dropped on the surface of the IDAM and the S. Typhimurium cells in the samples were captured by the antibody on the IDAM. This resulted in impedance changes which were measured and displayed with the LabVIEW software! An equivalent circuit of the immunosensor demonstrated that the largest change in impedance was due to the electron-transfer resistance. The equivalent circuit showed an increase of 35 % for the electron-transfer resistance value compared to the negative control. The calibration result indicated that the portable impedance immunosensing system could be used to measure the standard impedance elements and it had a maximum error of measurement of approximately 13 %. For pure culture detection, the system had a linear relationship between the impedance change and the logarithmic valueof S. Typhimurium cells ranging from 7.6x10' to 7.6*106 CFU (50 yl)~l. The immunosensor also had a correlation coefficient of 0.98, and a high specificity for detection ofS. Typhimurium cells with a limit of detection (LOD) of 102 CFU (50 nl)'1. The detection time from the moment a sample was introduced to the display of the results was 1 h. To conclude, the portable impedance immunosensing system for detection of S. Typhimurium achieved a LOD that is comparable with commercial electrochemical impedanceinstruments. The developed impedance immunosensor has advantages in portability, low cost, rapid detection and label-free feature showing a great potential for in-field detection offoodborne pathogens.