AUTOMATED PARALLEL DERIVATIZATION STRATEGY WITH BROAD METABOLITE COVERAGE COUPLED TO SWATH/MS DATA ACQUISITION FOR QUALITATIVE AND QUANTITATIVE ANALYSIS

摘要

Diversity in metabolite polarity and electrospray ionization efficiency challenge the development of a single method. Chemical derivatization can significantly improve chromatographic retention time and MS response. We propose a workflow including an automated fast parallel derivatization of amines, phenols, aldehydes, alcohols and ketones followed by QUAL/QUANT SWATH/MS analysis for broad metabolite coverage.