IMPACT OF CENTROIDING AND DIFFERENCES IN DATA PROCESSING ON QUANTIFICATION USING HIGH-RESOLUTION MASS SPECTROMETRY

摘要

The improved performance of High-Resolution MS (HRMS) systems triggers more (bio)analysts to switch from triple quad MS to HRMS for quantitative analysis. The impact of differences in data handling by different software packages, the impact of centroid or continuum data as well as the use of theoretical or measured accurate mass on the selection of the optimal narrow mass extraction window was studied.