ANALYSIS OF HOST CELL PROTEINS IN ANTIBODY PREPARATIONS USING PASEF

摘要

In the arena of biotherapeutic analysis, the analysis of host cell proteins (HCP) at the ppm level is critical. ELISA is currently the gold standard for QC applications, but the advantages of mass spectrometry are abundant. We show how PASEF (parallel accumulation and serial fragmentation), as implemented on the timsTOF PRO QTOF, can be applied to HCP analysis for highly sensitive detection with enhanced data quality.