Characterisation of immunoassay antibody-antigen interactions: ion mobility mass spectrometry as a potential tool

摘要

These results demonstrate the potential for IMS-MS to distinguish the variety of conformers present in protein reference standards and discriminate between immunoassay antibodies known to bind to different epitopes. In particular IMS-MS can be used to highlight the specific conformations an antibody interacts with, data not obtainable from conventional MS approaches, to further understand the specific measurand of each immunoassay approach.