Exploring the effect of DMSO as a mobile phase additive for improving protein identification and SWATH acquisition by NanoLC/MS/MS

摘要

NanoLC/MS/MS is the current method of choice for high sensitivity identification of proteins, e.g. for the discovery of biomarkers in plasma or other biological fluids. It has recently been demonstrated that solvents like DMSO can increase electrospray ionization efficiency and improve sensitivity. In this paper we will discuss optimization of the concentration of DMSO, the effect on chromatography, and the improvements on proteomics applications.