Untargeted analysis of reactive aldehydes produced by lipid peroxidation using selective derivatisation and detection by LC/HRMS.

摘要

Diets rich in red or processed meat were recently associated with an elevated risk of colorectal cancer (CRC) in some epidemiological studies. One of the major hypotheses involves dietary heme iron which could promote CRC by favouring lipid peroxidation. In particular, peroxidation of polyunsaturated fatty acids (PUFA) can lead to several decomposition products among which aldehydes and more specifically γ-hydroxy-α,β-unsaturated aldehydes have received attention due to their toxicity. Aldehydes such as 2(E)-4-hydroxynonenal (HNE) and 2(E)-4-hydroxy-hexenal (HHE) formed from respectively ω6- and ω3-PUFA, are known to be cytotoxic and genotoxic and may play a promoting role in CRC development, by selecting precancerous cells.