Treatment myocardial infarction by bone marrow autologous stem cells CD 133+

摘要

Objective: to study efficacy and safety of autologous stein cells of patients after macro focal myocardial infarction.Subjects: 30 patients with a diagnosis of ischeinic heart diseases, myocardial infarction (deferred Q-niyocardial infarction without significant complications barred from 3 to 6 months) were selected. Among these patients, 15 of them were treated with the standard protocol of treatment (control group), the 15 others were transplanted with their bone marrow stem cells. Bone marrow was taken as standard puncture of the sternum under local anesthesia. Stem cells CD133 (SC-CD133) were isolated by density gradient centrifugation using Ficoll followed by immuno-magnetic separation. Isolated cells of patients with coronary artery disease were injected infra-arterial into the coronary arteries under angiography in the average dose of 5 ml of suspension containing 0,8-1,5 million and they received 0.8mg estradiol/day for 60 days.Methods: Clinical examination, laboratory testing, ECG examination. Tomography was carried out with radiophannacological preparation (RPP) 99mTs-MIBI.Results: Results of clinical examination of patients revealed an improvement after 3 months and 6 months in both groups of patients. Key indicators of hemodynamic of heart were recorded before and after 6 months the introductions of autologous stem cells in the table.Results of patients with transplantation of autologous stem cells.As can be seen from the table, the ejection fraction of left ventricle in 6 months after the introduction of stem cells and significantly increased substantially. A similar dynamics was observed and end-diastolic volume of the left ventricle, which had a clear tendency to normalization.Conclusions: 1) Our method of stem cell transplantation is safe enough and does not increase mortality as a consequence of heart disease. 2} Treatment of autologous stem cells significantly improved key indicators of heart hemodyuarnic.