Proteomic analysis of wastewater cultured C. reinhardtii: Comparing protein expression for enhanced phycoremediation and biomass production

摘要

PCA biplot and Manova on the raw PCA score (P=0.0081) showed a significant difference in protein expression at proteome level based on Arabidopsis TAIR 10 functional descriptions. Counts of 92 differentially expressed proteins found functional enrichment in 56 KEGG metabolic pathway. Wastewater cultures showed continued photosynthetic and biosynthesis activity; control showed utilization of carbohydrate stores and recycling of amino acids. Of the proteins related to nitrogen metabolism and assimilation identified in this experiment, 5 were found to be significantly differentially expressed. Differentially expressed proteins and functional enrichment between culture condition suggested further research into elucidating the important role of post-transcriptional and post-translational regulation of protein expression and metabolism in microalgae could enhance wastewater treatment and biomass/biofuel production.