Sample Displacement Chromatography (SDC) for Differentiating Glycoforms on Human Glycoproteins for Cancer Biomarker Discovery

摘要

Proteins can be fractionated by sample displacement chromatography using LEL serial affinity chromatography set (SACS) to select glycans according to their binding affinity. The great advantage of this approach is that it allows the fractionation of glycoforms using single lectin SACS. This will be particularly useful in determining biomarkers in which the disease-specific feature is a unique glycan, or a group of glycans. Displacement phenomena are concluded to be a significant component of the separation mechanism in heavily loaded lectin affinity chromatography columns. This further suggests that care must be exercised in sample loading of lectin columns to prevent analyte displacement with nonretained proteins.