Serialized nanoparticle-mediated enrichment and profiling of the saliva proteome

摘要

We introduce a novel, highly automatable approach to serially enrich low-molecular weight proteins for discovery mass spectrometry of salivary biomarkers. Our detergent-assisted, optimized protocol employing serial nanoparticle fractionation and tandem digestion increases unique protein identifications and can be aptly applied to discovery-based experiments in saliva.