The p53 tumor suppressor protein bears a substantial number of post-translational modifications (PTMs), which are believed to comprise a combinatorial code regulating the stability, subcellular localization, and transcriptional activity of p53 as part of a stressor-specific cellular response [1]. Despite thorough characterization of PTM on active and inactive p53 within the context of various cellular stressors by targeted bottom-up MS/MS [e.g. 2, 3], the number of constituent proteoforms or relative degree of PTM abundance within any p53 population has yet to be determined.
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