Liquid extraction surface analysis mass spectrometry for protein analysis directly from Escherichia coli and Staphylococcus epidermidis

摘要

Mass spectra of E. coli K-12 and Staphylococcus sp. contain distinct protein signals corresponding to proteins unique to each species The overall reproducibility of the protocol is satisfactory; minor differences observed in samples subjected to cold storage are consistent with the expression of stress proteins The technique shows potential as a tool for top-down proteomic analysis of microorganisms.