Method development on a MALDI Orbitrap platform to achieve high throughput in situ DDA analysis via multiplex mass spectrometric imaging

摘要

- The multiplex-DDA-MSI approach is superior to traditional linear-DDA-MSI since this setup allows a continuous full MS scan in every raster position while obtaining DDA scans in subsequent spiral steps surrounding the main full MS step. - We introduced the concept of fractionating m/z range into multiple segments in multiplex-DDA-MSI acquisition to create a pseudo fractionation before DDA scans. This novel setup significantly improves the efficiency and coverage of precursor selection in MALDI MSI DDA. - With the precursor provided via our methodology, we made further improvements in the MS/MS acquisition by incorporating inclusion/target list and the combination of HCD/CID fragmentation into our multiplex-DDA-MSI setup, which enhanced the identification efficiency.