Quantitative LC-MS/MS analysis of polyamines and their metabolic precursors in lung tissue

摘要

In this study we developed a sensitive LC-MS/MS method enabling efficient analysis of polyamines and their amino acid precursors in lung tissue samples. The Scherzo SM-C18 LC column gave the best overall performance. Analytes were better retained on the Diamond Hydride column, but the Scherzo produced superior peak shape and thus the best sensitivity. Due to extremely high endogenous levels of the analytes (especially ARG and ORN) in lung tissue and the unavailability of a true blank matrix, highly diluted homogenate (1:50,000) served as calibration curve matrix.