Dried blood spot analysis of endogenous levels of β-hydroxy-β-methylbutyratein human blood using dual stable labelled isotopic standards

摘要

This is the first description of a direct and an LC-MS based confirmatory determination for β-hydroxy-β-methylbutyrate in whole blood using the dried blood spots (DBS) technique. It is also the first reported use of stable-labelled surrogate analyte to achieve quantitation of β-hydroxy-β-methylbutyrate in whole blood. The use of dual isotopically labelled standards allows for the determination of "endogenous" concentrations of β-hydroxy-β-methylbutyrate in wholeb lood. The use of the DBS technique and the stable-labelled surrogate ~(13)C-HMB analyte will facilitate further exploratory studies.