Improved sensitivity of large scale proteomic analyses using High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS)

摘要

FAIMS enhanced the identification of low abundance peptide ions that remained undetectable by conventional LC-MS/MS analyses. Reduced background contribution and decreased co-fragmentation of isobaric precursor ions provided higher number of quantifiable peptide with higher accuracy of fold change measurements. The analytical advantages obtainable using LC-FAIMS-MS/MS were exploited in quantitative proteomics to determine changes in protein expression and protein SUMOylation of HEK293 cells exposed to heat shock.