Validation of Liquid and Gas H2S Concentrations Via Derivatization-LC/MS and GC/MS for Determining Skin Transport

摘要

We have replicated a derivatization method for free H_2S and demonstrated UPLC/MS detection of physiologically relevant levels of H_2S in reference buffer and physiological buffer. Results suggest that MS~(Λ3) scans may be required to eliminate isobaric interferences for quantitation in our protocol. We also see a chlorinated bimane and are attempting to determine its source and effect on the quantitation of H2S in our procedures. We have detected H_2S permeation from a liquid to gas through a rat skin membrane. Further experiments to optimize the balance between time evolution and diffusion through a synthetic or skin membrane for gaseous H2S are needed. Overall progress toward a simultaneous measurement of H_2S levels on both liquid and gas sides of a skin to determine skin transport is encouraging.